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KMID : 0379119970250040330
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Korean Journal of Mycology
1997 Volume.25 No. 4 p.330 ~ p.339
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Purification and Characterization of Endo - polygalacturonase Produced by Plant Pathogenic fungus , Botrytis cinerea
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Abstract
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Botrytis cinerea T91-1 has shown to produce at least four different polygalacturonases in a liquid medium containing citrus pectin as a carbon source. One of the enzymes, its molecular weight was estimated as 37 kDa by denatured polyacrylamide gel electrophoresis, was purified by a series of procedures including acetone precipitation, ion exchange, heparin affinity, and reverse phase column chromatographies. By viscometric analysis, the enzyme was revealed as an endo-polygalacturonase. The enzyme activity was inhibited by divalent cations such as Ca^(2+), Co^(2+), and Cu^(2+). Km and Vmax for polygalacturonic acid hydrolysis were 0.33 ㎎/㎖ and 28.6 nM/min, respectively. The optimum temperature for enzymatic activity was 55℃ and the enzyme showed optimal pH values between 4.0 and 4.5. The enzyme was stable up to 12 hours in the range of pH 4 to 7 and at the temperature below 30℃. Amino acid sequence from N-terminal up to 6 amino acids determined by Edman degradation showed little homology with polygalacturonases from fungi and plants.
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